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The effects of testosterone in humans and other vertebrates occur by way of two main mechanisms: by activation of the androgen receptor (directly or as DHT), and by conversion to estradiol and activation of certain estrogen receptors. Free testosterone (T) is transported into the cytoplasm of target tissue cells, where it can bind to the androgen receptor, or can be reduced to 5α-dihydrotestosterone (DHT) by the cytoplasmic enzyme 5α-reductase. DHT binds to the same androgen receptor even more strongly than T, so that its androgenic potency is about times that of T. The T-receptor or DHT-receptor complex undergoes a structural change that allows it to move into the cell nucleus and bind directly to specific nucleotide sequences of the chromosomal DNA. The areas of binding are called hormone response elements (HREs), and influence transcriptional activity of certain genes, producing the androgen effects.

The next document issued was a proposed rule dated June 17, 1994, which states, “FDA is issuing a notice of proposed rulemaking in the form of an amended tentative final monograph that would establish conditions under which OTC topical health-care antiseptic drug products are generally recognized as safe and effective and not misbranded. FDA is issuing this notice of proposed rulemaking on topical antimicrobial drug products after considering the public comments on that notice and other information in the administrative record for this rulemaking. FDA is also requesting data and information concerning the safety and effectiveness of topical antimicrobials for use as hand sanitizers or dips.” [70] In the 1994 update to the rule, TCS was effectively removed from the drug category which made it available for use in consumer products. [71]

DDE has been shown to be toxic to rats at  mg/kg. [4] DDE and its parent, DDT, are reproductive toxicants for certain birds species, and major reasons for the decline of the bald eagle , [5] brown pelican [6] peregrine falcon , and osprey . [7] These compounds cause egg shell thinning in susceptible species, which leads to the birds’ crushing their eggs instead of incubating them, due to the latter’s lack of resistance. [8] Birds of prey , waterfowl , and song birds are more susceptible to eggshell thinning than chickens and related species , and DDE appears to be more potent than DDT. [7]

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